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  • Gluten Guardian 4.0 is a blend of 6 plant-based proteolytic enzymes that includes an enzyme clinically proven to help break down gluten — as well as three enzymes that break down starches and sugars.
  • Gluten Guardian 4.0 is specifically designed to support the digestion of gluten or casein, found in wheat and dairy.
  • Even if you eat gluten-free, most restaurant kitchens do not separate gluten-containing foods. This means there’s always the risk of gluten exposure anytime you eat out.



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  • Gluten Guardian 4.0 is a blend of 6 plant-based proteolytic enzymes that includes an enzyme clinically proven to help break down gluten — as well as three enzymes that break down starches and sugars.
  • Gluten Guardian 4.0 is specifically designed to support the digestion of gluten or casein, found in wheat and dairy.
  • Even if you eat gluten-free, most restaurant kitchens do not separate gluten-containing foods. This means there’s always the risk of gluten exposure anytime you eat out.
Frequently Bought Together

Gluten Guardian 4.0 is a one-of-a-kind blend of digestive enzymes targeting gluten, and is also designed to optimize dairy digestion.

  • Supports the digestion of hard-to-break-down gluten and dairy casein
  • Specifically designed to support digestion from high-carbohydrate meals
  • Can help relieve occasional bloating, gas, and indigestion from high-carbohydrate meals with three enzymes that break down starches and sugars
  • Improves digestion and absorption of nutrients
  • Supports muscle building with a blend of six plant-based proteolytic enzymes that are active across all acidity/alkalinity levels
  • Free of fillers, artificial colors, flavors, and preservatives
  • Non-GMO, Soy-free, dairy-free
  • 100% plant-based and in vegetarian capsules
  • Trace minerals provide cofactors for the enzymes and support healthy glucose uptake into cells


Protease, Peptidase, Serratipeptidase, Aminopeptidase, Bromelain, Fungal Amylase, Alpha Galactosidase, Glucoamylase, Lactase, Phytase, Invertase, Pectinase, Hemicellulase, Cellulase, Beta Glucanase, Lipase, Fungal Lipase, Lipase (Yeast), Lipase (Food Grade)

Other Ingredients: Hypromellose (Capsule), Rice Fiber

Gluten Guardian 4.0 Benefits

  1. Ensures Digestion of Gluten and Casein

    Gluten is a family of proteins found in wheat, whereas casein makes up 80% of the protein found in dairy. These proteins are difficult to digest because they are high in the amino acids proline and glutamine, which our body's natural digestive enzymes don’t recognize. As a result, they’re likely to remain undigested which may lead to occasional gut discomfort.

    Gluten in particular can open the gut barrier through the zonulin pathway, exposing the content inside the gut to your immune system. In people predisposed for autoimmunity or allergies, the leaky gut from gluten may trigger or worsen the disease. Incomplete digested gluten fragments can also get absorbed into the bloodstream and create opioid-like effects.

    Most dairy in North America have A1 casein, which is a common food allergen. Incompletely digested casein fragments may have opioid-like activities, as mother nature designs milk to help babies bond with their mothers. However, beyond infancy, A1 casein is a strong gut irritant that can irritate and inflame the gut, and slow down gut movement by acting like opiates.

    Gluten and dairy can cause leaky gut, allowing the incomplete fragments to enter the bloodstream. Some people with compromised blood-brain barriers may experience the opiate effects from these foods. Not surprisingly, people with food addictions and emotional eating often turn to gluten and dairy. Studies suggest that some people with neurologic or mental health conditions may find their nervous system better balanced without gluten and dairy.

    If you are healthy and experience minor sensitivity with gluten and dairy, it is important to take a gluten-digesting enzyme to ensure that they are 100% digested if you do eat them.

    If you have sensitivity after after eating gluten, you may need to adopt a gluten free diet. However, a digestive enzyme supplement that targets gluten may help with minor exposures. Keep in mind, however, that Gluten Guardian 4.0 is not intended to treat or prevent any disease.

  2. Enjoy Occasional High-Carb Refeed Meals and Treats Without the Bloat from Gluten and Dairy

    If you are on a restrictive diet or reaching a plateau with your weight loss program, a refeed meal allows you to enjoy your favorite foods and jumpstarts your metabolism. Even if you’re not dieting, you can enjoy your favorite treats with ease.

    The common problem is that eating a lot of carbs, sugar, and gluten may cause digestive upsets and inflammation. Therefore, it is important to supplement with digestive enzyme supplements targeting gluten and carbohydrates. Gluten Guardian 4.0 is designed to ensure that your digestion is optimal and helps lessen digestive upset from these refeed meals or treats.

  3. Take Advantage of Your Cheat Meal for Muscle Building

    Your cheat or refeed meals are a way to spike your hormones to get glucose and amino acids into your muscle cells and replenish your glycogen stores. If you do the refeed right, you will experience better workout during the first few days after your refeed.

    Also, some people use holiday meals and social events as refeeds to maximize muscle gain, fuel their workouts, and minimize fat gain. In order to maximize muscle building and minimize inflammation, your protein digestion and absorption have to be optimal.

    Gluten Guardian 4.0 can help ensure optimal digestion, minimize undigested gluten and casein, and promote a balanced immune response to food.

  4. Reduces Post-Meal Fatigue and Brain Fog

    Being tired and brain-fogged after meals are signs that your gut is working hard to digest your meals. A meal should simply take away your hunger, not put you to sleep. Gluten Guardian 4.0 digestive enzymes help break down the difficult-to-digest gluten and casein, and also provides overall support for your digestive system. As a result, you will feel both satiated and energized after your meals.

  5. Helps With Gluten and Dairy Exposures

    Gluten Guardian 4.0, can significantly reduce more gluten peptides than either enzyme alone .

    Gluten Guardian 4.0 is not intended to treat any conditions and individual responses may vary widely. Remember to work with your healthcare professional to decide what’s best for you.

  6. Helps With Leaky Gut and Food Sensitivities

    Leaky gut, or intestinal permeability, allows partially digested foods and bacterial toxins to reach the bloodstream and stimulate the immune system. Many people with leaky gut develop food sensitivities and allergies, gluten and dairy products being two of the most common culprits.

    Optimizing your overall digestion and ensuring that gluten and casein are completely digested can support your gut barrier health. These may also help mitigate food sensitivities.

How Does Gluten Guardian 4.0 Work?

Gluten Guardian 4.0 supports digestive function, especially for high-carb meals. It contains full-spectrum protein digestive enzymes specifically designed to target gluten, casein, and carbohydrates.

The most important enzyme in Gluten Guardian 4.0 is Peptidase DPP-IV, a potent gluten-digesting enzyme. It can also digest casein, a hard to digest protein that makes up 80% of the proteins in milk and dairy products. Therefore, Gluten Guardian 4.0 helps you fully break down gluten and casein into amino acids that your body can use. By fully digesting them, the enzyme helps minimize immune responses to incompletely digested gluten and casein.

When To Take Gluten Digestive Enzymes?

Take Gluten Guardian 4.0 with meals that contain gluten, dairy, or any high-carbohydrate meals.

Gluten Guardian 4.0 can also be taken on an empty stomach to harness the benefits of systemic protein-digesting enzymes. These include promoting exercise recovery, breaking down inflammatory proteins and scar tissues, and wound healing.

Cheat Day Stack

Gluten Guardian 4.0 Questions:

What is the recommended dosage for Gluten Guardian 4.0?

Take 3 capsules with water at the beginning of each meal for optimal digestion.

Do not exceed 9 capsules per day.

Consult your medical practitioner prior to starting any new supplement.

Can children take Gluten Guardian 4.0? If so, what is the recommended dosage?

Gluten Guardian 4.0 is safe for children. The recommended dose is 1 capsule for every 50 lbs.

Consult your medical practitioner prior to starting any new supplement.

I have a clean diet, why would I need to take Gluten Guardian 4.0?

Gluten Guardian 4.0 is great for the occasions that you want to indulge in gluten-containing foods or eat out, especially if you have some sensitivity to gluten or dairy. However, it is not intended to treat or prevent any disease.

I am already taking digestive enzymes. So why do I need to take Gluten Guardian 4.0?

Most digestive enzymes don’t contain enzymes that digest the most difficult to digest parts of gluten and casein, such as DPP-IV. Gluten Guardian 4.0 contains a high dose of DPP-IV along with other synergistic enzymes that optimize your digestion after consuming gluten-containing meals.

DPP-IV is a family of enzymes, some of which impact certain hormone production and neuronal functions in your body. Some food substances, including but not limited to partially digested gluten and casein, may inhibit enzymes in the DPP-IV family. Therefore, some people benefit from supplementing with digestive DPP IV on a regular basis to help reduce food-based DPP-IV inhibitors in the gut. However, keep in mind that Gluten Guardian 4.0 is not intended to treat any disease and you should consult your physician before use.

How many capsules are in a Gluten Guardian 4.0 bottle?

The Gluten Guardian 4.0 bottle contains 90 capsules.

Is it okay for pregnant or breastfeeding mothers to take Gluten Guardian 4.0?

Pregnant or breastfeeding mothers should consult a medical practitioner prior to starting any new supplement, diet, or training program.

Can I empty the contents of the capsule in a beverage?

The product is safe, but formulated for use as a Dietary Supplement and thus is labeled as such. We recommend consuming the product as is with your favorite beverage, per the directions of use, to achieve the noted benefits.

What is the shelf life for Gluten Guardian 4.0?

The shelf life for Gluten Guardian 4.0 is 2 years from the manufactured date, which can be found on the bottle.

How should you store Gluten Guardian 4.0?

Store Gluten Guardian 4.0 in a cool and dry place.

Gluten Guardian | Eat Gluten And Get Away With It
  • Made in USA
  • GMP Certified
  • Manufacturing COA

Product Information

Product Dimensions:

  • Cap diameter: 1.5 inches
  • Bottom diameter: 2 inches
  • Height: 4 inches
  • Weight: 2.2 ounces


Do not use if ulcer or gastritis are present.


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  • "BEST IN CLASS" guarantee: Our mission is focused on customer obsession. If after trying our products you don't feel we are the BEST IN CLASS, we will refund your money.
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BIOptimizers Was Born From Pain And Passion

Wade’s passion and mission for helping others optimize their health started when he witnessed his 23-year-old sister pass away from cancer. It was a heart-breaking experience that shaped his destiny. That’s when he realized that health was the most precious asset we have in this life.

Matt’s passion and purpose for helping others started when he helped his best friend lose 191 lbs in 18 months. He watched his friend’s entire life transform as a result. He went from never having a girlfriend to being married in just a couple of years.

Wade Lightheart

Wade Lightheart


Matt Gallant

Matt Gallant

Chief Executive Officer

Wade and Matt were both trainers working with pro athletes and other high performers. They both spent a decade in the gym helping people transform their health. Wade and Matt met in the gym, struck a friendship, and then started a business partnership that has been going strong since 2004.

That’s right, we started selling fitness programs, digestive enzymes and organic plant-based protein in 2004. Our obsession to build the best-in-class formulas and solutions just gets stronger. Our policy is, “If we can’t build the best product in that category, forget about it.”

We’re willing to go ALL IN on the formulas. What does this mean? Our average cost per bottle is 200% to 400% higher than most of our competitors. We use the optimal, effective doses of the most researched ingredients. We know that once you experience the results, you’ll be a fan of BIOptimizers for life.

Matt & Wade Matt & Wade
Matt & Wade Matt & Wade

Thank YOU for helping us become one of the fastest-growing companies in America - 2 Years in a row.

Thank you for trusting us with your health.


CEO/Co-founder of BIOptimizers


Scientific Studies
  1. Balakireva AV, Zamyatnin AA. Properties of Gluten Intolerance: Gluten Structure, Evolution, Pathogenicity and Detoxification Capabilities. Nutrients. 2016;8(10):644. Published 2016 Oct 18. doi:10.3390/nu8100644
  2. Brooke-Taylor S, Dwyer K, Woodford K, Kost N. Systematic Review of the Gastrointestinal Effects of A1 Compared with A2 β-Casein. Adv Nutr. 2017;8(5):739-748. Published 2017 Sep 15. doi:10.3945/an.116.013953
  3. Fasano A. All disease begins in the (leaky) gut: role of zonulin-mediated gut permeability in the pathogenesis of some chronic inflammatory diseases. F1000Res. 2020;9:F1000 Faculty Rev-69. Published 2020 Jan 31. doi:10.12688/f1000research.20510.1
  4. Pruimboom L, de Punder K. The opioid effects of gluten exorphins: asymptomatic celiac disease. J Health Popul Nutr. 2015;33:24. Published 2015 Nov 24. doi:10.1186/s41043-015-0032-y
  5. Shewry PR, Hey SJ. Do we need to worry about eating wheat?. Nutr Bull. 2016;41(1):6-13. doi:10.1111/nbu.12186
  6. Mkumbuzi L, Mfengu MMO, Engwa GA, Sewani-Rusike CR. Resistance is Associated with Gut Permeability Without the Direct Influence of Obesity in Young Adults. Diabetes Metab Syndr Obes. 2020;13:2997-3008. Published 2020 Aug 24. doi:10.2147/DMSO.S256864
  7. Jeanrenaud B, Rohner-Jeanrenaud F. Effects of neuropeptides and leptin on nutrient partitioning: dysregulations in obesity. Annu Rev Med. 2001;52:339-351. doi:10.1146/
  8. Salden BN, Monserrat V, Troost FJ, et al. Randomised clinical study: Aspergillus niger-derived enzyme digests gluten in the stomach of healthy volunteers. Aliment Pharmacol Ther. 2015;42(3):273-285. doi:10.1111/apt.13266
  9. Ehren J, Morón B, Martin E, Bethune MT, Gray GM, Khosla C. A food-grade enzyme preparation with modest gluten detoxification properties. PLoS One. 2009;4(7):e6313. Published 2009 Jul 21. doi:10.1371/journal.pone.0006313
  10. Fasano A. Intestinal permeability and its regulation by zonulin: diagnostic and therapeutic implications. Clin Gastroenterol Hepatol. 2012;10(10):1096-1100. doi:10.1016/j.cgh.2012.08.012
  11. Center for Drug Evaluation, Research. FDA Drug Safety Communication: FDA warns that DPP-4 inhibitors for type 2 diabetes may cause severe joint pain. Content current as of 06/23/2016. Accessed June 1, 2021.

*These statements have not been evaluated by the Food and Drug Administration. Probiotic supplements are not drugs and therefore are not intended to diagnose, treat, cure, or prevent any disease or health condition.